Lecture 27 - Genomics

December 5, 2013

Introduction

We began with a review of the Study Guide for the Final Exam, presented here.

Study Guide for Final Exam (12/12/2013)

Introduction

In general, the best preparation for the exam is to review the previous exams. Please see the exam keys with explanations for:

Exam 1
Exam 2
Exam 3

When reviewing the exams:

1. Compare your copy of Exams 1 - 3 with the answer key.
2. Lightly review the questions that you answered correctly.
3. Spend more time on the questions that you missed.

Make sure that you know how to solve the following kinds of problems:

• Human Pedigree Problems
• Codon Problems
• lac Operon Problems

Lectures covered

In addition, the exam covers the following lectures:

• Lecture 22: Recombinant DNA
• Lecture 23: Recombinant DNA
• Lecture 24: Genome Structure
• Lecture 25: Genome Structure
• Lecture 26: Genomics
• Lecture 27: Genomics

Here are some important highlights from these lectures, presented as questions.

Q1. How do I make cDNA from mRNA?

A1. Please see the lecture notes.

Q2. How does dideoxy (Sanger) DNA sequencing work?

A2. Please see the lecture notes.

Q3. What is a restriction fragment length polymorphism (RFLP)?

A3. Please see the lecture notes.

Q4. How do I make a targeted mutation (knockout) in a mouse?

A4. Please see the lecture notes.

Q5. What kinds of sequences are present in the human genome, and what is their relative abundance?

A5. Please see the lecture notes.

Q6. What is linkage disequilibrium?

A6. Please see the lecture notes.

Q7. What is a genome-wide association study?

A7. Please see the lecture notes.

Q8. In the ENCODE project, what kinds of sequences are associated with DNase I hypersensitivity?

A8. Please see the lecture notes, as well as these lecture notes.

Q9. In the ENCODE project, what do we learn from RNA-seq?

A9. Please see the lecture notes.

Q10. In the ENCODE project, what do we learn from ChIP-seq?

A10. Please see the lecture notes.